Cloning and identification of a novel human gene, CMLAP, involved in chronic myelogenous leukemia / 解放军医学杂志

ABSTRACT

Objective To study the rule of gene expression profiles of chronic myelogenous leukemia (CML), and to find new sensitive molecules which express specifically in peripheral blood mononuclear cells(PBMC) of CML patients, in order to further elucidate the potential molecular biological mechanisms leading to CML. Methods The expression of mRNA from PBMC of CML patients was compared with that of normal controls using a cDNA microarray. The bioinformatics analysis was used for every up- or down-regulated gene in CML. The new gene with unknown function and no homology to known genes in the database was confirmed, and electric polymerase chain reaction was conducted for the cloning of its full-length DNA in conjunction with Kozak rule and the exit of polyadenyl signal sequence. Sequence specific primers were designed to amplify the new gene from the mRNA of CML PBMC with the reverse transcription PCR (RT-PCR). Results Many genes commonly up- or down-regulated in CML cells were identified. Of these, we found a novel gene with unknown function with high expression in the patients cells. Its nucleotide sequence and corresponding protein-encoding amino acid had been determined, which contained 1 872nt and 624aa respectively. We named the new gene as human chronic myelogenous leukemia associated protein gene (CMLAP), and logged the sequence of the CMLAP gene into the GenBank with the accession number AY762229. Conclusion CMLAP gene expressed highly in CML PBMC was cloned and identified successfully by combining DNA chip technology and bioinformational technology. The gene was likely to play an important role in the disease and might serve as a molecular marker for CML. The findings will pave the way for the further study of the molecular mechanism of CML.