Preventing role and mechanisms of crude flavones of pueraria loblata on the damage of HUVEC cultured in high glucose medium / 中国药理学通报

ABSTRACT

Aim To investigate the protecting role and mechanisms of crude flavones of pueraria loblata(FP) on human umbilical vein endothelial cells(HUVEC) in high glucose medium.Methods ①HUVECs were incubated in 20% FCS RPMI-1640 culture medium containing 0,5,10,30,60,90,120 mmol?L~(-1) glucose for 48 hours.The proliferation of HUVEC was analyzed with MTT method(490 nm).② HUVECs were incubated in 20% FCS RPMI-1640 culture medium containing 0 or 30 mmol?L~(-1) glucose,either alone or in the presence of FP(final concentration 10~(-3),10~(-4),10~(-5),10~(-6) mol?L~(-1) respectively) for 48 hours.At the same time,30 mmol?L~(-1) mannitol was added.The proliferation of HUVEC was analyzed using MTT method.The cell cycle of HUVEC was analyzed using flowcytometry.The contents of SOD,NO and ICAM-1 in HUVEC supernatant were measured using test kits.Results HUVEC in 5 mmol?L~(-1) glucose medium proliferated well,but the proliferation of HUVEC in 10,30,60,90 and 120 mmol?L~(-1) glucose media was disturbed,showing a negative correlation.Proliferation of HUVEC was inhibited in 30 mmol?L~(-1) glucose,compared with that in 0 mmol?L~(-1) glucose and 30 mmol?L~(-1) mannitol(P