Changes of plasma malondialdehyde and superoxidase dismutase concentration in LPS-heat co-stressed rats / 第二军医大学学报

ABSTRACT

Objective:To investigate the effect of co-exposure to LPS and heat on plasma malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in rats.Methods: Male specific pathogen-free Wistar rats were randomly assigned to the following groups saline injection+normothermic control(C-Group),saline injection+heat exposure(H-Group),LPS injection+normothermic control(L-Group),and LPS injection+heat exposure(HL-Group).Rats in H-/HL-Group were exposed in a chamber at an ambient dry bulb temperature(Tdb) of(35.0?0.5)℃ and in C-/L-Group at an ambient Tdb of((26.0?)(0.5)℃.)Rats in L-/HL-Group were given an intravenous injection of LPS 10 mg/kg via tail veins to induce endotoxemia and in C-/H-Group were given an intravenous injection of 0.9%NaCl(10 ml/kg) via the tail vein.Mean arterial pressure(MAP) was continually monitored in all rats.Plasma levels of MDA and SOD activity were determined at 0,40,80,120 min after exposure.Results: There was significant difference in plasma MDA levels and activities of SOD between L-/H-/HL-Group and C-Group(P