Multiphication of calf rotavirus isolates in different cell cultures

ABSTRACT

Rotavirus strains isolated from 3 acute diarrhea calves at Ba Vi cattle farm were cultured and firstly cloned in African green monkey kidney (Vero) cells and African Rhesus monkey kidney (MA104) cells. After 3 times of passage, the calf rotavirus strains were secondly cloned and all of them were adapted in primary kidney cells of Macaca mulatta