Alphaviruses isolated from culex mosquitoes, 2001

ABSTRACT

In September – December 2001 period, from 4987 female Culex mosquitoes collected in Cat Que commune, Hoai Duc district, Ha Tay province, 4 viral species were isolated by clone C6/36 cells of Vero and Aedes albopictus. Among them, 2 species were alpha virus, 01VN61 virus was isolated from Culex tritaeniorhynchus and 01VN62 virus from Culex gelidus, both strains were adapted to 1-2 days age mice. By sequencing technique on E1 gene and 3 NCR gene area, 01VN62 virus was identified as Safiyama virus, a virus of alpha virus