Effects of YiqiWenyangHuoxue decoction on NF-?B in the myocardial remodeling of rat with chronic heart failure / 中华中医药杂志

ABSTRACT

Objective:To investigate the effects of YiqiWenyangHuoxue decoction on NF-?B in the myocardial remodeling of rat with chronic heart failure(CHF).Methods:Heart failure medel was established with ? ushing dose isoproterenol in rats and cardiac hypertrophy with angiotensin Ⅱ(Ang Ⅱ) stimulation 48h in myocardial cell.The model rats and cardiocytes with CHF were treated with YiqiWenyangHuoxue decoction,then cardiac index in model rats was measured;TNF-?,IL-18 were determined by ELISA;NF-?B mRNA evaluated with RT-PCR,and protein by Western-blot.Results:Compared with the control group,TNF-?,IL-18 concentration were significantly higher,as well as NF-?B mRNA and protein levels not only in myocardial tissues but also in cardiocytes of CHF groups.But those can be attenuated by YiqiWenyangHuoxue decoction.Conclusions:NF-?B plays important roles in the myocardial remodeling of CHF;and YiqiWenyangHuoxue decoction may reverse the myocardial remodeling through blockingNF-?B.