Research of Trametes Robinioplila on balance of Th1/Th2 in gene and protein level in children with asthma in remission stage / 中华中医药杂志

ABSTRACT

Objective:To explore the mechanism of Trametes Robinioplila on regulating the imbalance of Th1 and Th2 ratio in gene and protein level,and to find the theoretical basis of the fungus for treating asthma in remission stage.Methods:The PBMC from asthmatic children were divided into three groups(blank group,middle-dose group,large-dose group),and then treated respectively with Trametes Robinioplila(0g/L,3g/L,6g/L) and culture medium for 48 hours respectively.The expression of IL-4 and IFN-? mRNA in cellular precipitation were measured,and the expression of IL-4 and IFN-? in supernatant were measured too.Results:After PBMC cultured with Trametes Robinioplila for 48 hours,the expressions of IL-4 mRNA,IFN-?,IFN-?/IL-4 mRNA ratios and IFN-?/IL-4 ratios were significantly different(P0.05) between large-dose group and middle-dose group.Conclusion:Trametes Robinioplila markedly increased the expression of IFN-? and decreased the expression of IL-4 mRNA,then regulated the imbalance of IFN-?/IL-4 and Thl/Th2 ratios in asthmatic children in remission stage.