Effect of Lactobacillus Plantarum on expression of tight-junctional molecules in rat hepatocytes during common bile duct ligation / 肠外与肠内营养

ABSTRACT

Objective: To investigate the effects of oral Lactobacillus Plantarum supplementation on apoptosis of liver and tight junction of hepatocytes in an experimental model of obstructive jaundice.Methods: Wistar rats were randomly divided into five groups sham-operated;bile duct ligation;bile duct ligation +LP(0.2 g/5 mL/day);bile internal drainage;bile internal drainage+LP.Hepatocyte apoptosis was quantitated using TUNEL assay.Transmission electron microscopy,RT-PCR and Western blot were used to observe tight junction associated proteins.Results: Compared to the sham-operated,a significant increase in apoptosis(P