Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-566666
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore the effect of uremic serum on apoptosis in human umbilical vein endothelial cells(HUVECs) and the intervention by recombinant human erythropoietin(rhEPO) in this process.Methods From Dec.2008 to Apr.2009,10 uremic patients and 10 healthy volunteers were enrolled in this study.HUVECs were divided into 3 groupscontrol group(including 10% healthy serum medium),uremic group(including 10% uremic serum medium) and rhEPO treatment group(rhEPO at 5,10 or 15 U/ml was added to 10% uremic serum medium).After 24 hous's intervention,cell apoptosis was evaluated by TUNEL assay,and reactive oxygen species were detected by colorimetry.Results HUVECs apoptosis index and ROS level were higher in the presence of uremic serum than those of healthy serum(P
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Third Military Medical University
Ano de publicação:
2003
Tipo de documento:
Artigo