Detemination of Icariin and Flavonoids in Co-Wuzi Granule by HPLC and UV / 中草药

ABSTRACT

An HPLC method for the quantitative determination of icariin and an UV method for the determination of flavonoids in Co-Wuzi granule Preparation were developed. Results showed that the amount of icariin was 0. 050%, RSD=0. 72%, and the amount of flavonoids was 0. 120%, RSD=3. 8%. The HPLC method was very effective in the separation of active principles,suitable for the quantitative determination of single component in compounded TCM preparations,while the UV method may be used for the identification of active parts of TCM preparations.