Inhibition effect of insulin on cardiocyte apoptosis and its mechanism in diabetic rats / 解放军医学杂志

ABSTRACT

Objective To investigate the inhibition effect of insulin on apoptosis of myocardial mitochondria in type 2 diabetic rats and its anti-apoptotic mechanism through its action on the mitochondria.Methods Type 2 diabetes mellitus (T2DM) was reproduced in male Wistar rats by intraperitoneal injection of streptozotocin (STZ,25mg/kg) and feeding with a high-fat diet.Twenty-two rats were randomly divided into three groupsthe early treatment group (IE,n=7),the late treatment group (IL,n=7) and the diabetic group (DM,n=8).Another eight rats were chosen to constitute the control group.Novolin 30R was hypodermically injected into the rats in IE group in the first week and in IL group in the 4th week.Rats in DM group and control group were subcutaneously injected with an equivalent volume of normal saline.All groups were treated for eight weeks.At the end of the experiment,SOD,MDA,GSH levels,apoptotic index,mitochondrial membrane potential (?m),active oxygen and the changes in myocardial ultrastructure were compared among different groups.Results Compared with control group,blood glucose and HW/BW were higher (P