TOMOGRAPHIC ANALYSIS OF HUMAN RETINA BY LASER SCAN MICROSCOPY / 解剖学报

ABSTRACT

Using transmitted and reflected light confocal laser scan microscopic methods, we have studied the optical tomograms starting from outer layers to inner layers of an unstained human retina whole mount. The rod-structures which were located at the first superficial tomogram of the retina, had a width of 1.5 ?m and their center-to-center distance of 1-1.3 ?m. Toward the inner tomogram, there were the cone-structures which had a width of 3-3.2 ?m on their basal portion and their center-to-center distance of 5-11 ?m. The density for the rod-shape structures was 443 954 unit/mm~2 and the cone-shape structures was 20 128 unit/mm~2. We have discussed the characteristics of tomograms obtained from the laser scan microscope and its application in clinical uses.