Expression of the extracellular domain of human CD7 in Escherichia coli / 免疫学杂志

ABSTRACT

Objective　To get the antigen of CD7.Methods　 The extracellular domain of human CD7 was cloned from a plasmid containing the full length of human CD7 cDNA and expressed in pinpoint-xa3 prokaryotic system. Results　 Analysis with SDS-PAGE and Western-blotting showed that the expressed protein could bind to the anti-CD7 mAb specifically and is about 30 000 u in molecular weight. Conclusion　 These results paved the way for preparing anti-CD7 engineering antibody.