DISTRIBUTION OF HYALURONAN IN MACROPHAGES ANDEFFECTS OF HYALURONAN ON ADHESION AND MIGRATION OF THE CELLS / 解剖学报
Acta Anatomica Sinica
; (6)1954.
Article
em Zh
| WPRIM
| ID: wpr-572001
Biblioteca responsável:
WPRO
ABSTRACT
Objective To examine location of hyaluronan(HA) in macrophages and effects of HA on adhesion and migration of macrophages and explore regulating mechanism of HA. Methods HA of macrophages was labeled with aggrecan.Location of intracellular and extracellular HA of the cells was viewed using optic microscope and confocal laser scanning microscope.Effects of HA on adhesion and migration of macrophages were examined by adhesion assay and migration assay. Results Macrophage synthesized HA.HA located mainly on the membrane and in premeter and perinuclear area of the quiescent cells.In the moving cells,most of intracellular HA located in pseudopodia,tail and perinuclear area.HA was rich on the surface of the pseudopodia and tail of the cells.HA on the surface of macrophages and HA substratum increased cell adhesion and migration,addition of free HA into the medium decreased cell adhesion.Conclusion There are characteristics of HA distribution in macrophages.HA synthesized by macrophages and HA substratum promoted adhesion and migration of macrophage,while free HA reduces cell adhesion.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Acta Anatomica Sinica
Ano de publicação:
1954
Tipo de documento:
Article