cDNA microarray analysis of gene expression in acquired methotrexate-resistant of human choriocarcinoma / 中华妇产科杂志
Chinese Journal of Obstetrics and Gynecology
; (12)2001.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-572284
Biblioteca responsável:
WPRO
ABSTRACT
Objective To identify the molecular components involved in acquired methotrexate-resistance of human choriocarcinoma. Methods Methotrexate-resistant cell line(JAR/MTX) was derived from JAR cell line by exposed to intervally and progressively increasing higher concentration of MTX. cDNA microarray analyses of JAR/MTX and its parental cell line JAR were performed. Results JAR/MTX was established after one year with stable resistance. Its resistant index to MTX was 7.3. Nine genes were differential expressed between JAR/MTX and JAR cells. INSR,SLC1A3,SAT,HBB,and FLJ12443 were underexpressed and HS1,TXNRD1,TAGLN2,and EEF2 were overexpressed in JAR/MTX cells. Conclusion The cDNA microarray system showed that several alterations of gene expression were present in acquired methotrexate-resistance of human choriocarcinoma.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Obstetrics and Gynecology
Ano de publicação:
2001
Tipo de documento:
Artigo