TLC Fingerprinting Analysis of Total Glycosides of Paeony (TGP) / 中药新药与临床药理

ABSTRACT

Objective To establish TLC fingerprint of TGP for assessing the quality consistency of batch- by- batch of commercial product. Methods Silica gel 60 precoated plate (Merck) was used. Solvent system included chloroform- ethyl acetate- methanol- formic acid (40 ∶ 5 ∶ 10 ∶ 0.2); ∶ 5 % vanillin- sulfuric acid reagent was used as visualizing reagent and the heating temperature at 80 ℃ . Results Consistency among ten batches of TGP was demonstrated by TLC fingerprint image and the profiles generated by digital scanning. Conclusion TLC fingerprint of different batches of TGP confirms that TLC is also a powerful tool for developing chromatographic fingerprint, it can be used as a complementary technique for HPLC. 