Enhancing accumulation of indole alkaloids in Catharanthus roseus leaves / 中草药

ABSTRACT

Objective To study the effect of three kinds of reagent ethrel, Aspirin, tryptophan on the accumulation of medicinal component in Catharanthus roseus leaves. Methods To treat these potplants of C. roseus in green house with the three reagents in different concentrations, respectively. Then, two days later, the contents of catharanthine and vinblastine in leaves were determined by RP-HPLC. Results The three reagents can obviously improve the accumulation of indole alkaloids, including catharanthine and vinblastine in C. roseus leaves. Their optimum treatment concentration was finally comfirmed. Conclusion These three kinds of reagent apparently promote the accumulation of total indole alkoloids in the leaves of C. roseus.