Clinical significance of KiSS-1 and matrix metalloproteinase-9 expression in trophoblasts of women with preeclampsia and their relation to perinatal outcome of neonates / 中华妇产科杂志

ABSTRACT

Objective To investigate the role of KiSS-1 and matrix metalloproteinase(MMP)9 in trophoblasts in the pathogenesis of preeclampsia and their relation to perinatal outcome of neonates. Methods RT-PCR and western blot analyses were used to detect the MMP-9 and KiSS-1 expression levels in trophoblast of 40 patients with preeclampsia (15 cases of mild and 25 cases of severe preeclampsia)(preeclampsia group) and 20 cases of term pregnancy (normal pregnancy group) and their correlations with symptoms and perinatal outcome of neonates were analyzed. Results (1) The KiSS-1mRNA and metastin expression levels in trophoblasts of preeclampsia group were 1.73?0.24 (A value) and (78.4?8.0) ?g/ 100 ?g total protein separately,those of mild preeclampsia were (1.50?0.15) and (72.4?6.9) ?g/ 100 ?g total protein , and severe preeclampsia were (1.87?0.20) and (83.52?3.57) ?g/100 ?g total protein , which were all significantly higher than those of normal pregnancy group [1.24?0.25, P