Isolation of Rat Marrow Mesenchymal Stem Cells and Effect of Conditioned Medium on Its Proliferation / 广州中医药大学学报
Article
em Zh
| WPRIM
| ID: wpr-575725
Biblioteca responsável:
WPRO
ABSTRACT
【Objective】To establish a simple,rapid and practical method for the in-vitro isolation,purification and proliferation of rat marrow mesenchymal stem cells(MSCs).【Methods】Rat MSCs were isolated and purified by differential adhesion method.The effects of conditioned medium on the morphologic characteristics and growth of rat MSCs were observed.【Results】Rat MSCs were effectively purified by differential adhesion method,and grew well with good proliferation and normal morphology in conditioned medium.【Conclusion】Differential adhesion method and conditioned medium are effective for the purification and culture of rat MSCs.
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Guangzhou University of Traditional Chinese Medicine
Ano de publicação:
2000
Tipo de documento:
Article