HPLC fingerprint analysis of Jiketing Granules / 中成药

ABSTRACT

AIM: To establish the fingerprint analysis method of Jiketing Granules(Fructus Forsythiae,Radix Scutellariae,Radix Bupleuri,etc) by HPLC-UV.METHODS: Aanalysis was performed on an alltima C_(18)(4.6 mm?250 mm,5 ?m) column with a acetonitrile-0.1% acetic acid gradient.Detection time was 55 min.The flow rate was 1.0 mL/min.The montoring wavelength was changed.The colunm temperature was at 30℃. RESULTS: 21 peaks were separated on HPLC fingerprint in Jiketing Granules. CONCLUSION: The method is reliable,accurate and can be used as a quality control for Jiketing Granules.