Establishment of fingerprint of Cortex Eucommiae and its mutual mode by HPLC / 中草药

ABSTRACT

Objective To establish the fingerprints for analysis of Cortex Eucommiae by HPLC. Methods The column of Lichroshper C18(250 mm?4.6 mm, 5 ?m) was used. The mobile phase consisted of methanol-0.1% phosphoric acid with gradient elution. The detective wavelength was 230 nm, the column temperature was 30 ℃, and the flow rate was 1.0 mL/min. Results The fingerprint consisted of 11 common peaks. The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs from different habitats were compared by Similarity Evaluation System for Chromatographic Fingerprint of CMM (Version 2004A), and the range of similarity for ten balches of Cortex Eucommiae 0.574—0.958. The standard HPLC fingerprint of Cortex Eucommiae was established too. ConclusionThis method is accurate and reliable, and provides a scientific basis for the quality control of Cortex Eucommiae.