Comparative proteomics analysis of laryngeal squamous cell carcinoma

Jianrong ZHOU

ABSTRACT

Objective:

To study the differential expression proteins of laryngeal squamous cell carcinoma tissue and paired normal tumor adjacent mucosa epithelia tissue.

Methods:

The total proteins of laryngeal cancer tissue and paired normal tumor-adjacent mucosa epithelial tissue were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).Coomassie brilliant blue dyeing.The differential expression proteins were analyzed by using ImageMaster 2D analysis software. Then identified by peptide mass fingerprint(PMF)based on matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS)and database searching.

Result:

We obtained well-resolved,reproducible 2-DE patterns of laryngeal cancer and normal mucosa epithelia1.Differential protein spots were defined as spots in 2-DE gels.33 proteins were preliminarily identified,among which 22proteins were upregulated in laryngeal cancer tissue,while 11 were decreased significantly. PMF maps of 10 proteins which were upregulated in laryngeal cancer tissue were obtained by MALDI-TOF-MS. The typic peptide masses were searched in the Mascot database. Eight proteins were preliminanily identified.

Conclusion:

We established the expression maps of proteomics in laryngeal cancer and normal mucosa epithelia1.There were significant differences among proteome of laryngeal cancer and normal mucosa epithelia1.These proteins may be potential diagnostic markers and therapeutic targets for laryngeal cancer.And is valuable for studying mechanisms of laryngeal carcinogenesis.