Determination of syringin and betaine in Qianglining Tablet by HPLC / 中成药

ABSTRACT

AIM: To establish the HPLC method for determinating syringin and betaine in Qianglining Tablet(Radix et Rhizoma seu Caulis Acanthopanacis senticosi,Fructus Lycii). METHODS: A Hypersil C_18 column was used for the determination of syringin.The mobile phase for syringin was methanol-0.1 mol/L acetic acid (24∶76),and the detection wavelength was at 270 nm.A Hypersil NH_2 column was used for the determination of betaine.The mobile phase for betaine was acetonitrile-water(25∶75),and ELS detector was used. RESULTS: The linear range of syringin and betaine were 0.121 6-0.608 0 ?g(r=0.999 9) and 0.660 8-3.964 8 ?g(r=0.999 1),respectively.The average recoveries of syringin and betaine were 99.09%(RSD=2.13%) and 96.34%(RSD=1.31%),respectively. CONCLUSION: The method is simple,accurate,and can be used for the quality control of Qianglining Tablet.