Effect of MSCs Transplantation Combined with Qidantongmai Tablet on Myocardial Cell Apoptosis and Heart Function in Acute Myocardial Ischemia Rats / 中国中医药信息杂志

ABSTRACT

Objective To observe the effect of marrow mesenchymal stem cells(MSCs)transplantation combined with Qidantongmai tablet and simple MSCs transplantation on myocardial cells apoptosis and heart function in acute myocardial ischemia rats.Method Rat MSCs were isolated by density gradient method,and then cultured and amplified to the third passage labeled with BrdU.16 SD rats were divided into two groups randomly,transplantation combined with Qidantongmai tablet group and simple transplantation group.The isochoric of suspension of Qidantongmai tablet(2 g/kg)and NS were administered to SD rats by gastrogavage respectively for 14 days.The left anterior descending coronary artery of rat was ligated to establish animal models of acute myocardial infarction.The MSCs labeled with BrdU were implanted intramyocardially into the infarct area.After 3 hours,the heart function were detected by an echocardiographic system(HP 5500 system),equipped with a 12 MHz probe.After 4 weeks,the heart function were rechecked and MVD were detected by immunofluorescence,myocardial cells apoptosis were detected by TUNNEL.Results Heart function were improved in both groups,and was more significant in MSCs transplantation combined with Qidantongmai tablet group.Apoptotic index were decreased and the MVD of ischemic zone were increased in transplantation combined with Qidantongmai tablet group than simple transplantation group.Conclusion MSCs transplantation combined with Qidantongmai tablet could improve heart function of acute myocardial ischemia rats,decrease apoptosis of myocardial cells,increase the MVD of ischemic zone,the therapeutic effect were more significant than simple MSCs transplantation.