Content Determination of Ferulic Acid in Compound Prescribe Motherwort Herb Capusle by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To estblish a HPLC method for content determination of ferulic acid in compound prescribe Motherwort Herb capusle. Method The column ZORBAXSB-C18 was used with Acetonitrile-0.2% phosphoric acid aqueous solution (12∶88) as mobile phase, and detective wavelength was 274 nm, the flow rate was 1.0 mL/min. Result Within the range of 0.8～13 ?g, ferulic acid presented a fine linear relationship (r =0.999 99, n =6). The average recovery was 103.0% (RSD=0.4%, n=5). Conclusion The method is suitable, accuracy is high and reproducibility is good, recovery is high, and can control the quality of compound prescribe Motherwort Herb capusle.