Anoxia-reoxygenation up-regulates transcription of TGF?_1 gene in human umbilical veins endothelial cell line / 重庆医科大学学报

ABSTRACT

Objective:To investigate the influence of anoxia-reoxygenation on the transcription of TGF?1 gene in Human umbilical veins endothelial cell line(ECV304). Methods:Anoxia-reoxygenation model of Human umbilical veins endothelial cell line was established. TGF?1 mRNA was half-quantitatively analyzed by a modified RT-PCR. Results:The transcription of TGF?1 mRNA was up-regulated significantly in anoxia-reoxygenation group compared with the control group. Conclusion: anoxia-reoxygenation may up-regulate the transcription of TGF?1 gene in human umbilical veins endothelial cell line.