HPLC fingerprint analysis of Marsdenia tenacissima / 中成药

ABSTRACT

AIM:To establish the method of fingerprint analysis of Marsdenia tenacissima by HPLC-UV. METHODS: Analysis was performed on an Alltech C_(18)(4.6 mm?250 mm,5 ?m) column with the acetonitrile-(0.1%) acetic acid gradient.Fingerprint was finished in 55 min. The flow rate was 1.0 mL/min.The monitoring wavelength was at 328 nm. The column temperature was 30 ℃. RESULTS: 15 peaks were separated on HPLC fingerprint in Marsdenia tenacissima analyzed for chlorogenic acid,caffeic acid and part of derivatives. CONCLUSION: The method is reliable,accurate and can be used as a quality control method for Marsdenia tenacissima.