Determination of Osthol in Compound Shechuang Tincture by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish the determination method for osthol in Compound Shechuang Tincture. Methods Hypersil ODS column was used and methanol-water (70∶30) was used as a mobile phase. The flow rate was at 1.0 mL/min and the detection wave-length was at 322 nm. Results Osthol had a good linear relationship between 0.018～0.126 ?g (r =0.999 9). The average recovery was 98.55% and RSD was 1.45%. Conclusion This method is simple, rapid and accurate. It can be applied to determining osthol in Compound Shechuang Tincture.