Characterization and in vitro permeation of nasal mucosa evaluation of inclusion complex of cholic acid-hydroxypropyl-?-cyclodextrin / 中草药

ABSTRACT

Objective To improve the permeation of nasal mucosa by preparing the cholic acid-hydroxypropyl-?-cyclodextrin(HP-?-CD) and characterize the inclusion complex.Methods The inclusion complex was characterized by using phase solubility,scanning electron microscope(SEM),Fourier transform-infrared(FT-IR),differential scanning calorimetry(DSC),and superconducting NMR;In an effort to investigate the effect of HP?-CD on cholic acid,the studies of in vitro permeation of nasal mucosa was performed.Results Phase solubility curve was AL type,K=564.30 L/mol,and the characterization de-monstrates the formation of inclusion complex;The permeability coefficients and steady state fluxes through ovine and porcine nasal mucosa were enhanced by the formation of inclusion complex with HP-?-CD about 1.46 and 1.44 times of the uncomplexed cholic acid,respectively.Conclusion The inclusion complex is formed and it could increase the permeability of nasal mucosa by preparing the cholic acid-HP-?-CD.