Quality control and evaluation of Radix et Rhizoma Gentianae from GAP production base / 中草药

ABSTRACT

Objective To establish method for the fingerprint of Radix et Rhizoma Gentianae using UFLC with the hierarchical cluster analysis.Methods ColumnShimadzu C18(3.0 mm?75 mm,1.7 ?m);mobile phaseAcetonitrile-0.4% phosphorylation(5.5∶94.5);flow rate0.8 mL/min;detection wavelength240 nm;temperature40 ℃.Results This method had a good linearity in the range of 0.154 8—1.858 mg/mL,and the average recovery was 102.09% with RSD of 1.68% for swertiamarin.It had a good linearity in the range of 0.322—5.152 mg/mL,and the average recovery was 103.80% with RSD of 1.11% for gentiopicroside.The RSD of precision and reproducibility were lower than 2.1%.The result of fingerprint studying was the same with the hierarchical cluster analysis.Conclusion This method can be used as standardized implantation and quality control of Radix et Rhizoma Gentianae.