A Study on Preparation and Clinical Application of Antithrombin Ⅲ Concentrate Ⅰ. Isolation and Purification of Antithrombin Ⅲ / 中国输血杂志

ABSTRACT

A batch affinily adsorption method with heparin-sepharose 4B gel has been described for preparation and purification of antithrombin Ⅲ from plasma. The me- thod is suitable for batch preparation. The processes of each batch are completed only in 3 days. The recovery of activity was 22.4-42.3% from 20 liters of plasma. By fur- ther purification, the recovery of activity and antigen was 26.3% and 20.3% respec- tively, the specific activity was 9.9U/mg. The purified AT-Ⅲ showed a single band on disc gel electrophoresis, and the levels of purity, biologic activity and the recov- ery of activity are similar to those of for- eign reports.