Interleukin-13 and interleukin-18 on the expression of nerve growth factor mRNA in bronchial asthma model of rats / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect of interleukin-13 and interleukin-18 on the expression of nerve growth factor mRNA in bronchial asthma model of rats.Methods:Four Wistar rats were sensitized and challenged with ovalbumin to establish an asthma model,and the rat splenic lymphocytes were isolated and cultured with ConA.The expression of NGF mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR),and were observed after the lymphocytes were exogenously added with IL-18 or IL-13.Results:The lymphocytes of the asthma model stimulated by ConA in vitro expressed NGF mRNA in a time-dependent manner.After the lymphocytes had been cultured with IL-13 for 12,24 h and 48 h,IL-13 upregulated the expressions of NGF mRNA in a time-dependent manner and all the NGF mRNA expressions were signif-icantly higher than the basal values at the same time(P