Mood Group Serological Identification of Monocolonal Antibodies Anti-M and Anti-N / 中国输血杂志
Chinese Journal of Blood Transfusion
; (12)1988.
Article
em Zh
| WPRIM
| ID: wpr-581666
Biblioteca responsável:
WPRO
ABSTRACT
The BALB/c mice were immunized with human O blood cells. Their spleen cells were fused with murine myeloma cells Ns-1 with PEG. The monoclonal antibodies were identified with human panelled cells. Two clones secreting monoclonal anti-M(2Al and 3F6)and another two clones secreting monoclonal anti-N(4E3 and 7G5)were identified. All these clones could consistently secrete IgM antibodies. In typing 300 different RBC samples,the specificity of antibodies were identical to those of commercial humen anti-M and anti-N typing sere reagent. Significantly, anti-N reagent 4E3 differed from anti-N reagent 7G5, which the former one couldn't be absorbed by M erythrocytes at all,while the latter one absorbed by M erythroeytes.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Blood Transfusion
Ano de publicação:
1988
Tipo de documento:
Article