Proliferation and Immunophenotypic Analysis of CD3AK Cells Derived from Lymph Node / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
; (6)1995.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-583072
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To study the propagation and phenotypes changes of killer cell (CD3AK cell activated by CD3 mAb in vitro.Methods:
Lymph nodes taken from lung cancer patient is dissociated into single cell suspension by mechanical method and cultured in culture medium added CD3 mAb and a little dose IL-2. We analyze cell immunophenotype by flow cytometry and proliferation by trypan blue exclusion test per 2 days.Results:
Immunophenotypic analysis showed that CD3AK expressing CD3, CD8, CD56, CD25 increased, and reached a peak value which is 2.33 times than before culturing in the 8 th day.Conclusion:
CD3 mAb added to the culture medium can obviously activate CD3AK cell and stimulate proliferation and keep its killer activity.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Cancer Biotherapy
Ano de publicação:
1995
Tipo de documento:
Artigo