Detection of Duchenne and Becker muscular dystrophy patients by DNA microarray / 临床神经病学杂志
Journal of Clinical Neurology
; (6)1997.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-583467
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore the efficient method in detection of DMD/BMD patients.Methods 18 deletion-prone exon fragments of DMD gene were amplified via molecular cloning. They were used as probes and were spotted on the slides treated with APES and poly-lysine together by manual operation to make microarray. In addition, fragments of ?-actin were used as positive contrast and those of pUC 19/EcoR I were used as negative. 30 DMD/BMD patients were detected for deletion in DMD gene with the microarray and 5 healthy people were done as normal control. Parts of the results were compared with PCR method.Results Different exon fragment deletion of DMD/BMD gene was detected in 21 patients by DNA microarray, and 10 of them were confirmed by PCR analysis.Conclusion DNA microarray assay is a convenient ,accurate and sensitive method in diagnosis of DMD/BMD patient.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Guia de prática clínica
Idioma:
Chinês
Revista:
Journal of Clinical Neurology
Ano de publicação:
1997
Tipo de documento:
Artigo