Isolation and identification of human anti-oxidized LDL autoantibodies / 临床检验杂志

ABSTRACT

Objective To isolate and identify human anti-oxidized low density lipoprotein (LDL) autoantibodies.Methods Oxidized LDL (Ox-LDL) autoantibodies were isolated from eight healthy subjects by affinity chromatography with using an Ox-LDL cross-linked Sepharose 4B column.Results Three elution peaks of Ox-LDL autoantibodies were washed out from the Ox-LDL column by NaHCO3,acetate and KCNS buffer in turn and collected respectively.Ox-LDL autoantibodies were isolated from all the selected healthy subjects.All the three elution peaks had good reactivity with Ox-LDL.Among them,the elution peaks by KCNS buffer had high protein level and strong reactivity with Ox-LDL.The types of these antibodies included IgG and IgM, and IgG was the main type.Conclusion The isolated Ox-LDL autoantibodies had strong reactivity with Ox-LDL.