Ca ~(2+)Concentration change in platelets activated by chemical agonist / 中华检验医学杂志

ABSTRACT

Objective To observe the Ca 2+ concentration changes in the platelets activated by chemical agonist in different study group treated with aspirin.Methods We measured the Ca 2+concentration in the platelets activated by different concentration of ADP, thrombin, collagen, arachidonic acid and 5-HT with the flowcytometer in normal health (n=20).We measured the Ca 2+ concentration in the platelets activated by ADP, collagen, arachidonic acid and 5-HT in myocardial infarction (n=20) and hypertension (n=20).We measured the Ca 2+concentration in the platelets activated by arachidonic acid in myocardial infarction (n=20) treated with aspirin.Results As the concentrations of ADP, thrombin, collagen, arachidonic acid and 5-HT increased, the Ca 2+concentration in the platelets increased.There was the relation between agonist concentration and Ca 2+ concentration.Activated by the same agonist, the Ca 2+concentration in the platelets showed more increase in myocardial infarction and hypertension than in normal health.The Ca 2+ concentration show less increase in myocardial infarction treated with aspirin than in normal health.Conclusion The procedure which chemical agonist activating platelets is related to Ca 2+ concentration.The activity of Ca 2+ channels in platelets is high in myocardial infarction and hypertension.Examining the Ca 2+ concentration in platelets is helpful to supervise the aspirin therapy in myocardial infarction.