Antifibrotic Effects of Phosphodiesterase (PDE) Inhibitor in Experimental Interstitial Fibrosis induced by Unilateral Ureteral Obstruction
Journal of the Korean Society of Pediatric Nephrology
; : 85-91, 2002.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-58636
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Phosphodiesterase (PDE) inhibitor increases the cellular content of cAMP, and cAMP suppresses connective tissue growth factor (CTGF) expression induced by TGF-beta1. Therefore, we investigated whether PDE inhibitor suppresses renal fibrosis without suppression of TGF-beta. MATERIALS ANDMETHODS:
Renal interstitial fibrosis was produced by ligation of left ureter in Sprague-Dawley rats. Cilostazol, a selective PDE3 inhibitor, and dipyridamole, a hybrid PDE5, PDE6, and PDE8 inhibitor, were provided in drinking water for 7 days. In addition to the Masson-trichrome score of renal tissue, the concentration of fibronectin and TGF-beta1 in renal tissue-conditioned media was measured by ELISA.RESULTS:
Masson-trichrome score and fibronectin concentration were significantly lower in cilostazol-treated group compared to the control group (P<0.05). Though dipyridamole treatment seemed to suppress the Masson-trichrome score and fibronectin concentration too, the decrements were not statistically significant. There was no difference in TGF-beta1 concentration among the groups.CONCLUSION:
A selective PDE3 inhibitor cilostazol suppresses renal fibrosis without alteration of TGF-beta expression.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ureter
/
Obstrução Ureteral
/
Água Potável
/
Fibrose
/
Ensaio de Imunoadsorção Enzimática
/
Fator de Crescimento Transformador beta
/
Fibronectinas
/
Ratos Sprague-Dawley
/
Dipiridamol
/
Fator de Crescimento Transformador beta1
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of the Korean Society of Pediatric Nephrology
Ano de publicação:
2002
Tipo de documento:
Artigo