sHLA-G1 inhibiting xenotransplantation rejection through degrading the releasing of porforin and granzyme by NK cells / 医学研究生学报

ABSTRACT

Objective: To study the effect of sHLA-G1 inhibiting xenotransplantation rejection through degrading the releasing of porforin and granzyme by NK92. Methods: The recombinant expression vector pcDNA3-sHLAG1 was transfected into the lymphoblastoid cell line LCL721.221 by the nucleofection methods.The expression of sHLA-G1 in the transfected LCL721.221 was detected by RT-PCR and DOT-ELISA technique.NK cell line(NK92) was used as NK effect cells and the porcine aortic endothelial cells line(PED) as targets.The inhibitive effects of the releasing of porforin and granzyme by NK92 was analyzed by the ?-hexosaminidase release assay.And the cytotoxicity of NK92 was analyzed by MTT methods. Results:sHLA-G1 conferred a significant degrading the releasing of porforin and granzyme by NK92,and protection PED against NK92 medicated lysis,and the rate of NK92 cell cytotoxicity was reduced to 25.5% in contrast to 71.2% in the control group(P