Gene determination of transfusion transmitted virus-like mini virus / 吉林大学学报(医学版)

ABSTRACT

Objective To detect the transfusion transmitted virus-like mini virus(TLMV) sequence gene in patients with chronic hepatitis and to study the TLMV infection in population.Methods Two sets of primers in the most conservative regions of Japan strains TLMV-CBD279 and CBD231 were designed to amplify TLMV templete extracted from sera of patients with chronic hepatitis B using PCR.PCR products were cloned into pGEMR-T vector and sequenced.Results The results showed that 72%of TLMV sequence isolated in China was identical to that in Japan,suggesting that there was TLMV infection in patients with chronic hepatitis B in China.The patients with hepatitis virus C and the healthy blood donor had the highest infectious rates of TLMV.Conclusion TLMV infection exists in patients with chronic hepatitis B in China.