Human osteosarcoma multidrug resistance-related protein identified by differential in-gel electrophoresis / 中国肿瘤生物治疗杂志

ABSTRACT

Objective:To analyze the differential protein expression between multidrug-resistant human osteosarcoma MG-63/DXR100 and its parental cell line by differential in-gel electrophoresis,so as to lay a foundation for exploring the mechanism of multidrug resistance(MDR) of osteosarcoma.Methods:Multidrug-resistant clone of human osteosarcoma MG-63 was established by stepwise exposure to increasing doses of doxorubicin(DXR).The total proteins of the above two cell lines were separated with two-dimensional electrophoresis.The proteins of differential expression(increased or decreased by more than 30%) were analyzed with image scanning and DeCyder software.Then they were identified in MALDI-TOF Pro and Mascot database.Results:Thirty proteins with differential expression were identified by proteomic analysis,and 18 of them were further identified by MALDI-TOF Pro.Five protein spots were successfully identified:matrix metalloproteinases 1(MMP1) related with tumor cell metastasis,alcohol dehydrogenase(ADH6) with function of detoxication,FERM domain containing protein 3(FRMD3) which belongs to anti-oncogenes and two agnoproteins composed of 128 and 300 amino acid residues.MMP1,ADH6 and the two agnoproteins were over-expressed in MG-63/DXR100 group,and FRMD3 expression was lower than that in the MG-63 group.Conclusion:Five proteins including MMP1,ADH6,FRMD3 and two agnoproteins have been found abnormally expressed in MDR osteosarcoma cells by differential in-gel electrophoresis;they might participate in MDR of osteosarcoma.