Depletion of human serum albumins by ethanol precipitation / 医学研究生学报
Journal of Medical Postgraduates
; (12)2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-592966
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To explore the effectiveness of ethanol precipitation in depleting the human serum albumin.Methods:
Serum samples from 8 healthy controls and 8 lung adenocarcinoma patients were treated with 100% ethanol to deplete the albumin.The results of the albumin depletion were analyzed by 1-D gel electrophoresis and albumin quantitation.Results:
After ethanol treatment,1-D gel images of the serum samples showed a near depletion of albumins a presence of low-abundance proteins.There was no difference in the 1-D gel images between the normal and lung adenocarcioma serum samples.The average concentrations of albumin in the ethanol-treated serum samples were(5.88 ? 0.35) g/L and(5.50 ? 0.53) g/L respectively in the control and lung adenocarcinoma groups.Conclusion:
Ethanol precipitation can deplete most of the human serum albumins and thereby assist us in further analysis of human serum proteomics.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Artigo