A comparison of the detecting methods on gene polymorphism of methylene tetrahydrofolate reductase / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 1003-1004,1007, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-594003
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish a method for the detection of methylene tetrahydrofolate reductase (MTHFR) 677 C→T mutation by real-time fluorescence PCR.Methods MTHFR 677 C→T mutation was detected in 300 healthy people by the technique of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and real time fluorescence PCR. Sequencing was performed to confirm the accuracy of detection results. Results Three genotypes (CC, CT and TT) were found with two methods in all the 300 people. There were no significant differences in genotype frequency and allele frequency between the two groups (P>0.05).Conclusion Real-time fluorescence PCR is an accurate and rapid method for prompt grouping of MTHFR gene locus.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
International Journal of Laboratory Medicine
Ano de publicação:
2008
Tipo de documento:
Artigo