Comparison of constructing tissue engineering cartilage with palylact acid/poly plycolic acid,acellular cartilaginous matrix,and polylactic acid/poly plycolic acid-acellular cartilaginous matrix scaffold in vitro / 中国组织工程研究

ABSTRACT

BACKGROUND:The polylactic acid/poly plycolic acid (PLGA) exhibits excellent biomechanical property with poor cell adhesion. However,acellular cartilaginous matrix had good cell adhesion and hydrophilia,which can mediate signal conduction and interaction among cells,yet the biomechanical property,is poor. PLGA-acellular cartilaginous matrix scaffold is a remedy for their shortcomings,which can be a newly scaffold materials. OBJECTIVE:To observe the ability of PLGA,acellular cartilaginous matrix,PLGA-acellular cartilaginous matrix scaffold in supporting the growth of porcine chondrocytes for cartilage tissue engineering. DESIGN,TIME AND SETTING:The comparative observation experiment was performed at the Laboratory of Shanxi Medical University from March to September 2008. MATERIALSThe average aperture of PLGA is from 100 -200 ?m,with porosity rate of 94%. The average aperture of the acellular matrix scaffold ranged 70-100 ?m,with porosity rate of 85%. The average aperture of PLGA-acellular matrix aperture is 100-300 ?m,and the porosity rate is approximate 90%. METHODS:The experiment was divided into three groups according to the scaffoldsPLGA,acellular cartilaginous matrix,and PLGA-acellular cartilaginous matrix group. The free chondrocytes isolated from porcine knee articular were seeded onto 3 kinds of scaffolds after cartilage amplification,followed by 8 weeks culture in vitro. MAIN OUTCOME MEASURES:The expression of type Ⅱ collagen was detected by immunohistochemistry. The contents of mRNA of type Ⅱ collagen were determined by RT-PCR. Meantime,the content of DNA was measured by Hoechst 33258 method. RESULTS:Chondrocytes grows vigorous on the PLGA-acellular cartilaginous matrix scaffold,which can maintain phaenotype after 8 weeks of culture,and the secretion of type Ⅱ collagen was superior to other 2 groups. Meanwhile,the content of DNA and mRNA of type Ⅱ collagen of PLGA-acellularcartilaginous matrix groups were greatest,followed by acellular cartilaginous matrix group,and the content of PLGA was smallest. The one-factor analysis of variance showed the difference had significant (P