Clonality Assay of Dysplastic Epithelial Lesions of the Stomach
Journal of the Korean Gastric Cancer Association
; : 129-135, 2001.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-59954
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Dysplasia or flat adenoma of the stomach is regarded as a precancerous lesion. However, the frequency and the evolutionary process of malignant transformation of gastric dysplasia are still debated. In order to see whether the lesion was a monoclonal or a polyclonal proliferation, clonality was assayed by X-linked HUMARA polymorphism. MATENRIALS ANDMETHODS:
DNA was extracted from the paraffin-embedded tissue of 16 consecutive cases of endoscopic biopsy, eight of which supplied both dysplastic and nondysplastic tissue for comparison. HUMARA was amplified by PCR with or without pretreatment with methylation- sensitive restriction enzyme, HpaII. The amplification products were electrophoresed on polyacrylamide gel and silver-stained.RESULTS:
Among the 16 cases, 13 cases were informative and 3 cases noninformative. Of the 13 cases, one case showed skewed lyonization, rendering 12 cases to be analyzed further. A monoclonal band pattern was noted in 2 cases, and a polyclonal band pattern in 10 cases. A review of the histopathologies of the monoclonal and the polyclonal cases did not reveal features discriminating the two groups.CONCLUSION:
These results suggest that gastric dysplasia is a disease entity heterogeneous in the genetic level, and many cases may be non-neoplastic.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Estômago
/
Biópsia
/
DNA
/
Adenoma
/
Reação em Cadeia da Polimerase
/
Inativação do Cromossomo X
Idioma:
Coreano
Revista:
Journal of the Korean Gastric Cancer Association
Ano de publicação:
2001
Tipo de documento:
Artigo