Effects of 5-Aza-CdR on growth of TPC-1 cell line and KLF4 expression / 中华内分泌外科杂志
Chinese Journal of Endocrine Surgery
; (6): 480-483,492, 2015.
Article
em Zh
| WPRIM
| ID: wpr-603079
Biblioteca responsável:
WPRO
ABSTRACT
Objective Methylation of anti-oncogene can be demethylated by related drugs which can help the inactivated gene to express again .This study aims to study the effects of the demethylating agent 5-Aza-2′-deoxycytidine on the growth of human thyroid papillary cancer cell line TPC-1 and mRNA and protein expres-sion of KLF4.Methods TPC-1 cells were treated with different concentration of 5-Aza-CdR.MTT was used to detect the influence of 5-Aza-CdR on cell proliferation .RT-PCR was used to detect mRNA and protein expression levels of KLF4.Results After being treated with 5-Aza-CdR for 24 hours, 48 hours, and 72 hours, the growth of TPC-1 cells was inhibited and the inhibition was in time and concentration depended manner .After treatment with 5-Aza-CdR, mRNA and protein expression levels of KLF 4 were increased, and the difference had statistical significance(P<0.05).Conclusion 5-Aza-CdR can inhibit the cell viability of TPC-1 cells through upregulat-ing KLF4 expression , which may provide experimental basis for 5-Aza-CdR in treating thyroid cancer .
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Endocrine Surgery
Ano de publicação:
2015
Tipo de documento:
Article