Determination of Luteolin-7-O-glucoside in Sedum Sarmentosum Bunge from Different Regions by HPLC / 中国药师
China Pharmacist
; (12): 584-585,586, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-603520
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To establish an HPLC method for the determination of luteolin-7-O-glucoside in Sedum Sarmentosum Bunge.Methods:
The chromatographic column was Waters SymmetryShield C18(250 mm ×4.6 mm,5μm). The mobile phase consis-ted of tetrahydrofuran-methanol-water-phosphoric acid (9 ∶17 ∶74 ∶0. 25). The flow rate was 1. 0 ml·min-1. The detection wave-length was 350 nm. The chromatographic column temperature was 35℃. The injection volume was 10 μl.Results:
The calibration curves were linear within the range of 5. 2-208. 0 μg · ml-1 ( r =0. 999 9 ) for luteolin-7-O-glucoside. The average recovery was 99. 12%(RSD=0. 94%,n=6).Conclusion:
The method is simple, rapid, accurate, specific and repeatable. It can be used for the determination of luteolin-7-O-glucoside in Sedum Sarmentosum Bunge.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2016
Tipo de documento:
Artigo