Development and evaluation of a rapid test strip for gB antibody detection of pseudorabies virus / 中国兽医学报
Chinese Journal of Veterinary Science
; (12): 1463-1467, 2017.
Article
em Zh
| WPRIM
| ID: wpr-606832
Biblioteca responsável:
WPRO
ABSTRACT
To evaluate the gB antibody level of pseudorabies virus (PRV) in swine,a rapid test strip was developed.In the strip,the expressed protein of gB was labeled with colloidal gold,the staphylococcal protein A (SPA) and swine anti PRV antibody were blotted on the nitrocellulose membrane for the test and control lines,respectively.The specificity and sensitivity of the strip were detected with standard positive,negative and immunized sera of PRV,the results indicated that the strip was high specificity and sensitivity.Field swine serum samples were tested by the new strip and commercial IDEXX PRV gB ELISA kit,simultaneously.The agreement rate of the two methods was 91.04%.Furthermore,the dipstick assay based on the strip is rapid (5 min),sensitive and easy to perform.This suggests that the new strip is an acceptable alternative for field diagnosis.
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1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Veterinary Science
Ano de publicação:
2017
Tipo de documento:
Article