Upregulation of miR-96-5p inhibits the proliferation of lung adenocarcinoma cell A549 / 实用医学杂志
The Journal of Practical Medicine
; (24): 2625-2628, 2017.
Article
em Zh
| WPRIM
| ID: wpr-611825
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore the effects of hsa-miR-96 to A549. Method A549 were transfected with miR-96 inhibitor/mimics and shRNA-mTOR. The proliferation were detected by MTT,the expression of p21, cyclin D1,p-4E-BP,S6K were detected via RT-PCR and WB. The luciferase assay were used to anaylse the target of miR-96. Result With or without metformin treated,up-regulated the expression of miR-96 could inhibit A549 proliferation,increase p21 expression and decrease cyclin D1 expression instead. The results may related with G1 arrest which induced by miR-96 up-regulation. The ratio of firefly fluorescence value/Renilla fluorescence value was lower than that in wild type NC or mutant group. Conclusion miR-96 target with mTOR to inhibit the growth of A549.
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1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
The Journal of Practical Medicine
Ano de publicação:
2017
Tipo de documento:
Article