miRNA expression between deep and moderate hypothermia circulatory arrest and its impact on intestinal protection / 中华胸心血管外科杂志
Chinese Journal of Thoracic and Cardiovascular Surgery
; (12): 226-229, 2017.
Article
em Zh
| WPRIM
| ID: wpr-615990
Biblioteca responsável:
WPRO
ABSTRACT
Objective To evaluate the miRNA change between hypothermia circulatory arrest at different temperature and its impact on intestinal protection.Methods Sixteen piglets were randomly(n =4) divided into four groups:deep hypothermia circulatory arrest (DHCA,18℃) group,moderate hypothermia circulatory arrest(MHCA,24℃) group,cardiopulmonary bypass(CPB) group and sham operation(SO) group.They were subjected to 80 min hypothermia circulatory arrest,305 min CPB or thoracotomy,respectively.Pick-and-mix custom miRNA real-time PCR panels were utilized to detect intestinal samples.miRNA expression between DHCA and MHCA were compared directly(DHCA vs.MHCA) and indirectly(DHCA/SO vs.MHCA/SO,DHCA/CPB vs.MHCA/CPB).Results Exposure to DHCA caused less intestinal miRNA dysregulation than MHCA.Besides,seven miRNAs(miR-122,miR-145-5p,miR-421-5p,miR-99a,miR-365-5p,miR-31 and miR-192)were differentially expressed between the two hypothermia circulatory arrest groups.Conclusion Better intestinal miRNA protection was provided by DHCA than MHCA.Intestinal miRNA were differentially expressed between hypothermia circulatory arrest at different temperature.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2017
Tipo de documento:
Article